Figure 2.

Cryosections of mouse trachea, showing reporter gene expression in the respiratory epithelium after gene transfection by BGTC/DOPE liposomes in vivo. (A and B) Detection of β-galactosidase expression in transfected cells located in the surface epithelium (A) and in submucosal glands (B) by X-Gal staining. (×400.) (C) Detection of β-galactosidase-expressing cells in submucosal glands by immunoperoxidase labeling using a monoclonal anti-β-galactosidase antibody. (×400.) (D) Detection of luciferase-expressing cells in submucosal glands by immunoperoxidase labeling with a polyclonal anti-luciferase antibody. (×400.) (E) Immunoperoxidase staining: negative control performed by omitting the primary antibody. (×400.)