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. 1997 Mar 4;94(5):1657–1662. doi: 10.1073/pnas.94.5.1657

Table 1.

Rate constants for association (kon) and dissociation (koff) of the complex between IL-4 variants and IL4-BP immobilized at a biosensor matrix

IL-4 variant kon × 10−6 M−1·s−1 koff × 103 s−1 IL-4 variant kon × 10−6 M−1·s−1 koff × 103 s−1


IL4 13 2.1
I5A 12 14 I5R 14 8.7
T6A 14 1.9 T6D 8.8 15
Q8A 16 2.5 Q8R 11 1.9
E9Q 8.7 270 E9K ND ND
E9A ND ND
I11A 11 2
K12S 12 1.9 K12E 7.3 1.5
T13A 8.4 7.1 T13D 7.6 0.85
N15A 15 2.3 N15D 12 1.7
S16A 16 1.9 S16D 11 1.5
E19A 18 1.7 E19R 12 1.6
R53Q 11 7.3
K77A 10 2.1 K77E 4.4 2
Q78A 11 2.2 Q78E 11 2.7
R81A 7.7 2.8 R81E 3.2 6.1
F82A 15 2.1 F82D 12 0.73
K84A 10 2.9 K84D 2.4 9.3
R85A 8.1 2.7 R85E 3.6 4.6
R88Q 7.3 140 R88D ND ND
R88A 8.3 760
N89A 12 27 N89R ND ND
W91A 11 6.1 W91D 5.8 8.5

The association and dissociation of IL-4 variants and IL4-BP immobilized at a biosensor matrix was measured in real time by means of a BIA2000 system. The rate constants represent mean values of 12–24 independent measurements performed with at least three different analyte concentrations. The mean standard deviation was 15% for the kon and 25% for the koff values. ND, not determined (only unspecific binding was found). 

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