Fig. 3.

C terminus LANA interacts with both F-box and WD40 domain. ICN was in vitro-transcribed and translated. (A and B) The ³⁵S-labeled products were incubated with GST as well as GST-F-box (A) or GST-WD40 fusion protein (B). (C and D) C terminus LANA was in vitro-transcribed and translated. The ³⁵S-labeled products were incubated with GST as well as GST-F-box (C) or GST-WD40 fusion protein (D). The pulldown products were electrophoresed on 10% SDS/PAGE gel, dried, and exposed to a PhosphorImager. Input control is 10% of total translation product. A schematic diagram of Sel10 is shown below the blots. Sel10 is a 540-aa protein, and the F-box motif and WD40 repeated are indicated.