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. 2007 Oct 1;104(41):16287–16292. doi: 10.1073/pnas.0703508104

Fig. 4.

Fig. 4.

LANA/ICN competition for Sel10 binding. (A) Seven hundred sixty-two to 1,162-aa truncations of LANA and ICN were in vitro-transcribed and translated. The 35S-labeled products were incubated with GST-Sel10. A fixed amount of ICN and increasing amounts of LANA were used. Pulldown products were electrophoresed on 10% SDS/PAGE gels, dried, and exposed to a PhosphorImager. Input controls of 10% for LANA and for ICN were run as well. (Right) A fixed amount of ICN and increasing amounts of luciferase were used. Pulldown products were electrophoresed on 10% SDS/PAGE gels, dried, and exposed to a PhosphorImager. The quantification of each band is shown at the bottom. (B) LANA/ICN competition for Sel10 WD40 domain binding. Seven hundred sixty-two to 1,162-aa truncations of LANA and ICN were in vitro-transcribed and translated. The 35S-labeled products were incubated with GST-WD40. The procedure is the same as that mentioned in A.