Table 3.
Parameters before refinement
| Source | ligand | LC(Z) | LC(X) | 50K(Z) | 50K(X) | S1 ϕ | RLC | ELC | TM | Symmetry |
|---|---|---|---|---|---|---|---|---|---|---|
| Skeletal | rigor | 2 | 4 | 0 | −6 | −3 | 0.46 | 0.7 | 0.6 | 54/25 |
| ADP | 2 | 12 | −4 | −14 | 0 | 0.5 | 0.7 | 0.56 | 54/25 | |
| Cardiac | rigor | −2 | 8 | 4 | −12 | −1 | 0.68 | 0.78 | 0.6 | 54/25 |
| ADP | 4 | 16 | 4 | −8 | −1 | 0.74 | 0.86 | 0.6 | 54/25 | |
| Gizzard | rigor | −6 | 14 | 4 | −12 | −5 | 0.74 | 0.84 | 0.58 | 54/25 |
| ADP | 6 | 14 | 2 | −16 | −11 | 0.56 | 0.66 | 0.48 | 54/25 | |
| IIA | rigor | −6 | 8 | 0 | −14 | −2 | 0.7 | 0.8 | 0.6 | 54/25 |
| ADP | 12 | 4 | 4 | −2 | −1 | 0.78 | 0.88 | 0.6 | 13/6 | |
| IIB | rigor | −6 | 6 | −6 | −24 | 1 | 0.72 | 0.82 | 0.58 | 54/25 |
| ADP | 12 | 4 | 2 | −8 | −1 | 0.8 | 0.88 | 0.6 | 13/6 |
The values obtained in the first step (before refinement) are listed here. RLC, ELC, and TM refer to the relative occupancy of regulatory and essential light chains and tropomyosin chain, respectively. Symmetry refers to that of F-actin that best explains the lattice sampling pattern of ALL's (determined by visual inspection of the diffraction pattern).