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. Author manuscript; available in PMC: 2007 Oct 2.
Published in final edited form as: Structure. 2006 Mar;14(3):577–587. doi: 10.1016/j.str.2005.11.015

Figure 4.

Figure 4

Steady-State Expression of AcrA and Mutants

Western blotting analysis with anti-AcrA antibodies of total cell protein (1 μg per lane) separated on an SDS-polyacrylamide (12%) gel. AcrA was expressed along with AcrB under control of the native acrAB promoter from various derivatives of pAHisB plasmid in E. coli AG100AX (ΔacrAB::kan ΔacrEF::spe). Steady-state expression of intact AcrA(1-397) (WT), AcrA L287M/L288M, AcrA F223M/L224M, AcrA F223M/L224M/L287M/L288M, and AcrA(1-312) is shown.