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Biochemical characterization of Pab-S chimeric protein. (A) FPLC gel filtration on a Superdex G-200 column of reduced (broken line) and oxidized (solid line) forms of the Pab-S molecule. V0, exclusion volume; VT, total bed volume. (B) Coomassie blue staining of reduced (lane 1) and nonreduced (lane 2) forms of Pab-S analyzed by 10–15% gradient SDS/PAGE.
