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. Author manuscript; available in PMC: 2007 Oct 2.
Published in final edited form as: Nat Neurosci. 2006 Sep 17;9(10):1247–1256. doi: 10.1038/nn1775

Figure 6. Netrin-1 gradient elicits asymmetric increase of β-actin across the growth cone.

Figure 6

Growth cones from stage 24 retinal explants were exposed to a gradient of netrin-1 for 5 minutes and then stained for β-actin. Fluorescence intensities of both sides of the growth cone were measured and the near/far ratios of the different groups were compared (f). In control condition, β-actin was expressed at similar levels on both sides of the growth cone (a). Netrin-1 gradient induced an asymmetric rise in β-actin QIF signal on the near side (b), which was blocked by CHX (c) and β-actin AMO (d). Netrin-1 did not cause β-actin asymmetry in growth cones grown on high laminin substrate (e). Images were pseudo-coloured (see colour bar in a). ** P = 0.003, *** P < 0.001, Kruskal-Wallis test. Scale bar 10 μm. Error bars s.e.m. Numbers inside bars indicate the number of growth cones analyzed.