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Daidzin is a mixed inhibitor of hamster liver ALDH-2 oxidation of acetaldehyde. Conditions: pH 9.5, 3 mM NAD+. (A) Replot of kinetic constants obtained from progress kinetic curves based on Eq. 3. The y-intercept is equal to 0 and hence β (Eq. 3) is equal to 0; the slope of the straight line is equal to αKi (0.65 μM), the noncompetitive inhibition constant. (B) Replot of kinetic constants based on Eq. 4; the slope of the straight line is equal to Ki−1 (23.8 μM).
