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. 1997 Mar 4;94(5):1680–1685. doi: 10.1073/pnas.94.5.1680

Figure 5.

Figure 5

Figure 5

Colocalization of D181A-PTP1B and endogenous EGFRs. COS 1 cells transfected with wild-type PTP1B (data not shown) or C215A (A and B) or D181A expression plasmids (C and D) were fixed with paraformaldehyde at 36 h after transfection and processed for immunofluorescence as previously described (24). Cells were incubated with anti-PTP1B FG6 ascites fluid (10) and anti-EGFR polyclonal serum (1964, kindly provided by G. Gill) at dilutions of 1/4000 and 1/500, respectively. Overexpressed PTP1B (A and C) and endogenous EGFR (B and D) were visualized with fluorescein-conjugated sheep anti-mouse and Texas red-conjugated goat anti-rabbit antibodies (Cappel), respectively.