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. 1997 Mar 4;94(5):1680–1685. doi: 10.1073/pnas.94.5.1680

Figure 6.

Figure 6

The analogous substrate-trapping mutant in PTP-PEST does not interact with the EGFR. This figure shows an anti-pTyr blot (RC20b) of precipitates prepared from lysates of COS cells that were transfected to express PTP1B, the C215A-PTP1B mutant (M1), the D181A-PTP1B mutant (M2), the GST fusions of these proteins, or PTP-PEST and the analogous mutants of it: C231S-PTP-PEST (M1) or D199A-PTP-PEST (M2). PTP-PEST was immunoprecipitated with mAb AG25 (27). The expression levels of the PTP-PEST proteins were similar to those for PTP1B.