Figure 5.

Changes in pairwise coupling energies as a function of the reaction coordinate of the allosteric transition of GroEL relative to the TT state. Coupling energies between Asp-41 with Thr-522 and between Glu-409 with Arg-501 in the TR state relative to the TT state, and in the RR state relative to the TR state, were calculated from the data in Figs. 3 and 4 using Eq. 2. The coupling energy in the RR state relative to the TT state, ΔΔG_int(RR-TT), was calculated from ΔΔG_int(TR-TT) + ΔΔG_int(RR-TR). Coupling energies were divided by seven to be for a single interaction.