Figure 4.

Inhibition by anti-β3xo oligonucleotide B24 of expression of α_1E currents and rescue of α_1E currents by coinjection of cRNA encoding the rat β1b subunit. Oocytes were injected with 50 nl of a solution containing 100 μg/ml of α_1E cRNA alone or in combination with 100 μg/ml β1b cRNA and the indicated concentrations of B24 oligonucleotide. Ca²⁺ channel currents were recorded 6 days after injection. The results presented on this figure were obtained with oocytes obtained from a single frog. Similar results but varying in the concentration of B24 needed to affect α_1E currents, were obtained in two other experiments. (A) Peak inward α_1E currents recorded from oocytes after a 250-msec test pulse from a holding potential of −90 mV. Injection of cRNAs and B24 oligonucleotide are indicated above the bars, which are means ± SEM of 4–5 oocytes. (B) Averaged G–V curves (mean ± SEM) of α_1E currents in oocytes coinjected or not with B24 and B24 plus β1b cRNA. The composition of B24 is given in Table 1.