Table 1.

Effect of oocyte β subunit antisense oligonucleotide on functional expression of coinjected calcium channel α₁ subunit

Injections		Ion channel activity,† frequency of success
α1 subunit cRNA	Oligonucleotide,* 20 nl per oocyte
α1C‡	—	20/22
α1C	B10 sense, 100 μM	6/6
α1C	B11 antisense, 100 μM	0/10
α1C	B11 antisense, 10 μM	0/14
α1E	—	27/29
α1E	B10 sense, 100 μM	10/10
α1E	B11 antisense, 100 μM	0/4
α1E	B8 antisense, 100 μM	3/16§
α1E	B24 antisense, 100 μM	0/12
α1E	B24 antisense, 10 μM	20/20¶
α1E	B11 antisense, 10 μM	1/9‖

^*Sense and antisense refer to coding strand of common sequences of Xenopus oocyte xo-28 and xo-32 cDNAs. B8, 5′TTGAGCC[A/T]GCCTCTCCACCTC; B10, 5′AGATTTCCTACATATCAAGGAGAA; B11, 5′GCACTCCTCATCCAGCGCTCCACAG; and B24, 5′TGAACCCACTTCTGAGTCTTCAAA. 

^†x of y oocytes from 2 to 3 frogs. 

^‡All α_1C experiments were performed with the ΔN60 mutant of α_1C lacking amino acids 1–60 at the N terminus. 

^§1–5% of control in the three positives. 

^¶16 ± 8% of control at 30 mV. 

^‖3% of control for the only positive.