Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Inhibition of rhodopsin phosphorylation by rhodopsin mutants. Rhodopsin was present at 0.1 μM concentration while the mutants were added at increasing concentrations up to 10 μM. Truncated rhodopsin (Rho-CT) was used as the control for inhibition of WT rhodopsin phosphorylation. The [32P]phosphate incorporation was measured by SDS/PAGE. Each assay (Materials and Methods) used 4 ng RK of standard specific activity.
