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. 1997 Mar 4;94(5):1721–1726. doi: 10.1073/pnas.94.5.1721

Figure 3.

Figure 3

Optimal conditions for stable complex formation of Gal80p and HA-Gal3p. Whole-cell extracts were prepared from yeast cells (NFG1) overexpressing Gal80p and HA-Gal3p grown in ESGlyLac. (A) Effect of varying concentrations of galactose on the formation of Gal80p/HA-Gal3p complex. Immunoprecipitation was performed in the presence of 2 mM ATP. Galactose was added to both binding buffer and washing buffer at the concentrations indicated at top. (B) Effect of varying concentrations of ATP on the formation of Gal80p/HA-Gal3p complex. Immunoprecipitation was performed in the presence of 10 mM galactose. ATP was added to both binding buffer and washing buffer at the concentrations as indicated at top.