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Effect of optical isomers of galactose on the formation of Gal80p/HA-Gal3p complex. Whole-cell extracts were prepared from yeast cells (NFG1) overexpressing Gal80p and HA-Gal3p grown in ESGlyLac. Immunoprecipitation analysis of extracts from these cells was performed in the presence of 2 mM ATP. Galactose, glucose, or mannose was added to both binding buffer and washing buffer at 10 mM.
