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Interaction between Gal80p and HA-Gal3p at the purified state. (A) One hundred nanograms of purified Gal80p (lane 1) or purified HA-Gal3p (lane 2) were electrophoresed on SDS/polyacrylamide gel followed by silver staining. (B) Purified HA-Gal3p (150 ng), purified Gal80p (50 ng), ATP (2 mM), and galactose (10 mM) were mixed in the binding buffer supplemented with 0.05% Tween 20. The formation of Gal80p/HA-Gal3p complex was analyzed by immunoprecipitation exactly as in Fig. 2.
