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Expression of G protein α and β subunits in heart membranes from Gαo+/+, Gαo+/−, and Gαo−/− mice. Gαo protein is absent and Gβ is reduced in ventricular membranes from Gαo−/− mice. Gαi, Gαs, and Gαq are unchanged. Shown are the bands of interest from representative immunoblots and the densitometric evaluation of the chemiluminescence signal.
