Figure 2.

bFGF and AT-III affinity chromatography of bulk Hep and HA-Hep. (A) Unfractionated [³H]Hep (•) or HA-Hep (□) were resuspended in PBS (pH 7.4) and then loaded onto a 1-ml bFGF affinity FPLC column equilibrated in PBS (pH 7.4). The column was eluted with a gradient of 0–2 M NaCl/10 mM phosphate, pH 7.4 (dashed line), at a flow rate of 0.5 ml/min. Each fraction (0.5 ml per fraction) was collected and radioactivity determined by liquid scintillation counting. (B) Unfractionated [³H]Hep (•) or HA-Hep (□) resuspended in PBS (pH 7.4) was loaded onto a 1-ml AT-III affinity FPLC column equilibrated in PBS (pH 7.4). The column was eluted with a gradient of 0–2 M NaCl/10 mM phosphate, pH 7.4 (dashed line), at a flow rate of 0.5 ml/min. Each fraction (0.5 ml per fraction) was collected, and radioactivity was determined by liquid scintillation counting.