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. 1997 Mar 4;94(5):1749–1754. doi: 10.1073/pnas.94.5.1749

Figure 2.

Figure 2

Reverse-phase HPLC chromatograms of dAdo adducts formed by alkylation with (±)-anti-BPDE or its racemic chlorohydrin derivative (trans-BPDCH). (A) Adducts formed with the epoxide in the absence of chloride. (B) Adducts formed with the epoxide in the presence of 175 mM NaCl. (C) Adducts formed with trans-BPDCH in the absence of chloride. (D) Co-injection of the samples used in A and C. Peaks in A are labeled with the enantiomer of BPDE from which the adduct was derived [(+), RSSR; (−), SRRS] and with the epoxide ring opening geometry (c = cis; t = trans). Adducts were formed at room temperature in 10 mM sodium cacodylate buffer (pH 7.0) containing 5% THF (vol/vol), and analyzed by reverse-phase HPLC using a 1:1 methanol/water mobile phase and fluorescence detection.