Figure 2.

Distribution of Na⁺ pump α subunit isoforms in freshly isolated, fixed MA myocytes. (A) Cell was labeled with PcSynA1 directed against α1. (B) Cell was labeled with PcSynA3 raised against α3. The “single focal plane” images were restored with CELLscan. (A and B Insets) Cell cross sections, perpendicular to the plane of the coverslip, at the positions indicated by the double arrows in Aa and Ba. The four consecutive cross sections are 0.25 μm apart. After these images were captured, the cells were treated with DiOC (Ab and Bb) to stain the SR (arrowheads) and mitochondria (arrows).