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A nuclear factor(s) binds specifically to the HIV-2 pets site. EMSA using Jurkat nuclear extracts with the HIV-2 pets site probe. The shifted band seen in lane 1 (indicated by the arrow on the left) can be competed away by the addition of unlabeled self-competitor (lanes 3 and 4), but not by a mutant version of the pets site oligonucleotide (lanes 6 and 7) or by unrelated HIV enhancer elements (lanes 7–13).
