Figure 2.
Purified recombinant DEK–(HIS)6 binds to the HIV-2 pets site in EMSA (lane 1). Addition of cold unlabeled pets oligonucleotide competed away the shifted band indicated by the arrow (lanes 3 and 4) but not when mutant or unrelated binding site oligonucleotides were added (lanes 5–12). The mutant oligonucleotide (mut-2) added in lanes 8–10, unlike mut-1 (lanes 5–7), did not have the downstream Elf-1 binding site mutated. In lane 13, human anti-DEK serum has been added to the reaction, leading to a supershift.