Table 3.
CAD copy number, specific aspartate transcarbamylase activity, and levels of PALA resistance in HT-1080 clones
| Cells | Amplification mode, FISH | Relative copy no., Southern | Relative ATCase activity* | PALA resistance, ID50, μM |
|---|---|---|---|---|
| Wild type | None | 1.0 | 1.0 | 10 |
| 30-9A | None | 1.0 | 1.6 | NA |
| 40-2A | None | 1.1 | 1.7 | 75 |
| 40-6P | None | NA | NA | 60 |
| 50.4 | Ladder | 2.6 | 3.9 | 75 |
| 50-4P | Isochromosome | NA | NA | 50 |
| 20-1A | Ladder | NA | 2.1 | NA |
| 50-6P | Isochromosome | NA | 2.2 | 75 |
| 60-1B | Isochromosome | NA | NA | 100 |
NA, not analyzed; ATCase, aspartate transcarbamylase.
*
The value for 30-9A is an average of duplicate assays (1.5 and 1.7); the values for 40-2A (1.7, 2.1, and 1.2) and 50.4 (3.3, 6.0, and 2.4) are averages of triplicate assays; 20-1A and 50-6P were assayed only once.