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Ii is sialylated in BFA-treated cells and in cells overexpressing wt rab6 and rab6 Q72L. HeLa cells were transfected, pulsed, and chased as described in the legend to Fig. 4. After immunoprecipitation, Ii immunoprecipitates were treated with (+) or without (−) neuraminidase. Asterisks (∗) point to the shift in mobility of Ii caused by the removal of sialic acids. Note that both p31 and p33 are shifted down upon neuraminidase treatment.
