Figure 5 Western blot analysis of SPINK1 expression in conditioned media and cell lysates of transfected HEK 293T cells. Aliquots of conditioned media (5 μl) and cell lysates (10 μl; ∼3.5 mg/ml protein concentration) harvested 48 hours post‐transfection were subjected to polyacrylamide gel electrophoresis, transferred to Immobilon P membranes and detected with a commercial anti‐Glu‐Glu tag antibody (Abcam).