Figure 3.
Determination of Apoptosis by the TUNEL assay. MCF-7 and MDA-MB231 cells were exposed to 500nM JG-03-14 for 5 days and monitored for apoptosis utilizing the TUNEL assay. Continuous exposure to Taxol (1μM) was used as a positive control for apoptosis. Apoptosis was monitored in combined cytospins of both adherent and non-adherent cells. Data presented are from one of two experiments with similar results. A. Untreated MCF-7 cells. B. Taxol, 1μM. C. MCF-7 cells after three days exposure to JG-03-14. D. MDA-MBA231 cells after three days exposure to JG-03-14. Magnification was 10X.