Figure 2.

Apical secretion of the mutant (HB-Tyr146) lacking N-glycosylation from permanently transfected MDCK cells. MDCK cells expressing either the wild-type HBsAg (lanes 1 and 2) or the HB-Tyr146 mutant (lanes 3 and 4), in which the the unique Asn-146 N-glycosylation site was replaced by Tyr, were grown on filters in Transwell chambers and pulse–chase labeled. Densitometric analysis of the fluorograms of the immunoprecipitated proteins resolved in SDS/PAGE indicated better than 80% apical distribution in both cases.