Figure 2.

The SD of the NRPD1b CTD has a WG/GW repeat signature sequence. (A) Dot plot analysis of AtNRPD1b versus SoNRPD1b highlighting the divergent region of the CTD. Positions of the divergent and AGO4-binding regions are indicated in relation to a schematic representation of AtNRPD1b. The SD shows sequence identity to the N-terminal region of HsGW182. The WG motifs are indicated with blue dots. The RNA recognition motif (RRM) domain of HsGW182 is indicated in brown. (B) Relative distribution of the WG/GW/GWG motifs within the SDs of plant NRPD1b proteins. (C) Myc-AGO4 or HA-AGO2 extracts were applied onto equimolar amounts of GST and GST-based fusion protein beads, and bound proteins were detected by immunoblotting with anti-Myc and anti-HA antibodies. (D) Alignment of AtNRPD1b and HsGW182. The WG/GW repeats are highlighted in red.