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. 2007 Oct 15;21(20):2580–2592. doi: 10.1101/gad.1569307

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Copyright © 2007, Cold Spring Harbor Laboratory Press

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Figure 5. — Depletion of Rpf2, Rrs1, or rpL5 causes 66S preribosomes to accumulate in the nucleus. GAL-RPF2 (JWY7703), GAL-RRS1 (JWY7706), or GAL-RPL5 (JWY7696) strains expressing eGFP-tagged rpL25 were grown at 30°C in galactose medium to 3 × 10⁷ cells cells per milliliter. A second culture of each strain grown in galactose medium was shifted to glucose medium for 17 h to 3 × 10⁷ cells per milliliter. Nuclei were stained with DAPI, and ribosomes containing eGFP-rpL25 were detected by fluorescence microscopy.