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. 2007 Oct 15;21(20):2644–2658. doi: 10.1101/gad.435107

Figure 1.

Figure 1.

SIRT1 regulates the angiogenic activity of endothelial cells. (A) mRNA expression of SIRT1–SIRT7 in HUVECs as assessed by RT–PCR using the indicated primer pairs. GAPDH served as loading control. (B) Statistical summary of the SIRT expression profile as assessed in a microarray analysis of total RNA isolated from HUVECs. (C) Three-dimensional in vitro angiogenesis with collagen gel-embedded spheroids of solvent-treated, nicotinamide-treated (NAM, 5 mM), sirtinol-treated (100 μM), or resveratrol-treated (1 μM) endothelial cells. Cumulative length of all sprouts originating from an individual spheroid was quantified after 24 h. Representative micrographs and a statistical summary are shown. (D) HUVECs were transfected with different siRNAs targeting SIRT1, SIRT2, SIRT3, SIRT5, or a nonrelated scrambled control. A statistical summary of the cumulative sprout length after 24 h originating from individual spheroids in siRNA-transfected endothelial spheroids is shown. The mRNA expression of the SIRTs with deacetylase activity in siRNA-transfected HUVECs as assessed by RT–PCR using the indicated primer pairs is shown in the left panel. (E) The effects of SIRT1 gene silencing on the expression of mRNA transcripts for SIRT1–SIRT7 were assessed by RT–PCR using HUVEC RNA from SIRT1 siRNA-transfected endothelial cells.