Figure 6.

Dysregulated gene expression in SIRT1-silenced endothelial cells. HUVECs were transfected with a SIRT1-specific siRNA or a scrambled oligonucleotide siRNA (each n = 3). Total RNA was isolated after 24 h, and the gene expression profile was assessed with the Affymetrix gene chip expression assay. (A,B) Selected genes that were either down-regulated (A) or up-regulated (B) in SIRT1-silenced endothelial cells. (C) RT–PCR analysis of selected genes in scrambled and SIRT1 siRNA-transfected endothelial cells. (D) RT–PCR analysis of selected genes in lung endothelial cells freshly isolated from mice with the respective genotype. (E) Fli1 expression, as revealed by whole-mount in situ hybridization, is reduced in the ISVs (arrowheads) of SIRT1 morphants (SBmo-inj.) compared with that in the control embryos. The GFP signal visualized by fluorescence microscope in live embryos is weaker in SIRT1 morphants. (Panels a,b) Embryos 24 hpf, lateral view; Bar, 50 μm. (Panels c,d) Embryos 4 dpf, lateral view.