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. Author manuscript; available in PMC: 2008 Aug 1.
Published in final edited form as: Dev Cell. 2007 Aug;13(2):177–189. doi: 10.1016/j.devcel.2007.06.009

Figure 6. BR Treatment and Mutation of 14-3-3 Binding Site Increase Nuclear Localization of BZR2/BES1.

Figure 6

(A) 14-3-3λ interacts with BZR2 in yeast two-hybrid assays. pACT2 is the empty prey vector as a negative control.

(B) BL treatment increases the nuclear localization of BZR2. BZR2-GFP transgenic seedlings were grown on media containing 2 μM BRZ or 10 nM BL in the dark for 4 days, and BZR2-GFP subcellular localization was visualized by confocal microscopy. Numbers in each image show the average ratios between nuclear and cytoplasmic signal intensities and standard errors calculated from seven cells for each treatment. Error bars are ± standard errors of mean.

(C) YFP fusion constructs of BZR1, BZR1S173A, BZR2 or BZR2S171A were transiently expressed in tobacco leaves and subcellular localization of YFP was observed. Scale bar is 10 μm.