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. Author manuscript; available in PMC: 2008 Sep 18.
Published in final edited form as: Cancer Lett. 2007 May 31;255(1):135–144. doi: 10.1016/j.canlet.2007.04.005

Fig. 5.

Fig. 5

Enriched expression of a glial marker in lysates of pseudopodia harvested from the second specimen. Aspirated cells formed pseudopodia that were harvested as an urea-based lysate for comparision with unmigrated aspirated cellular material. Expression of GFAP (predicted molecular weight of 55 kDa) was increased in pseudopodia compared to unmigrated cells with GAPDH (predicted molecular weight of 36 kDa) present to indicate loading. Although lower molecular weight bands for GAPDH in lysates of unmigrated cell materials suggested the presence of degeneration, only the intact form of GAPDH was found in the lysates of pseudopodia. Lanes 1 and 4 with approximately equivalent amounts of intact GAPDH demonstrated a 9.43-fold increase of GFAP in pseudopodia compared to unmigrated cells. Hemoglobin in the lysates precluded measurements of their protein content. Almost all of the pseudopodial lysate obtained from the second specimen was loaded in the two lanes (3 & 4) shown.