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. 2007 Aug 23;26(17):3879–3887. doi: 10.1038/sj.emboj.7601816

Figure 1.

Figure 1

Substrate binding to PME. (A) Stereoview of the cartoon representation of the three dimensional structure of PME with bound hexasaccharide (compound II) shown in ‘stick' representation. Residues D199, R267 and D178A are also shown. The mutation D178A mutant was used to trap the Michaelis complex. (B) Surface representation of PME together with stick representation of hexasacharide II showing the shape and position of the substrate-binding cleft, the reducing end of the hexasaccharide is to the right of this Figure and the substrate would move through the binding site from right to left. (C) The four specifically methylated hexasaccharides used in this work. Compound I: R1=R2=Me, R3=H; compound II: R1=R2=H, R3=Me; compound III: R1=Me, R2=R3=H; compound IV: R1=H, R2=R3=Me. Pymol (DeLano, 2002) was used to produce this figure and also Figures 2 and 3.