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Steady-state kinetic analyses of mutant PMEs
| kcat (s−1) | Km (mm) | kcat/Km (s−1 mm−1) | |
|---|---|---|---|
| PME3937 wild-type | 450±12 | 0.13±0.02 | 3462 |
| Q153A | 232±19 | 2.2±0.4 | 106 |
| Q177A | 40±1 | 0.22±0.01 | 181 |
| D178A | — | — | — |
| V198A | 68±3 | 0.06 ± 0.01 | 1133 |
| D199A | — | — | — |
| R267A | — | — | — |
| W269A | — | — | — |
| T272A | 425±8 | 0.77±0.04 | 552 |
| M306A | 59±1 | 0.53±0.03 | 111 |
| The calculation of Km in mM assumes that all methylated GalAs are substrates and the degree of methylation of the pectin used (Sigma P9135) is 9%. | |||
