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. 2007 Sep 21;104(40):15805–15810. doi: 10.1073/pnas.0707628104

Fig. 3.

Fig. 3.

MiR-29s directly target DNMT3A and -B. (a) Results of the luciferase assay for DNMT3s expression after transfection with miR-29s in A549 cells. (b) (Upper) Assessment of expression of DNMT3A and DNMT3B mRNAs by qRT-PCR, after transfection of A549 cells with miR-29s or a negative control. (Lower) Silencing of miR-29s with antisense molecules (AS) induces increased expression of DNMT3A and DNMT3B mRNA. (c) Western blot of proteins extracted from A549 cells that were cotransfected with the GFP repression vectors for the DNMT3A and -B−3′-UTR plus miR-29s or scrambled (Scr) oligonucleotides. (d) miR-29b acts as an endogenous primer to retrotranscribe its predicted DNMT3B mRNA target. Black, DNMT3B cDNA (GenBank accession no. NM_175848); blue, cloned and sequenced cDNAs experimentally obtained (eight clones analyzed); red, deduced RNA sequences and corresponding miR-29b. The upper underlined black and blue nucleotides have no homology between target and experimental cDNAs. The lower underlined red nucleotides represent RNA sequence complementary to cDNAs that lack homology to the miR-29b sequence. Nucleotides in bold represent the PicTar-predicted match site.