Fig. 6.

Effects of miR-29s on tumorigenicity of A549 cells. (a) Growth curve of A549 cells transfected in vitro with miR-29s or scrambled oligonucleotide or mock-transfected. (b) Percentages of live cells were measured in A549 cells transfected with scrambled oligonucleotide or with miR-29s oligonucleotides (100 nM final concentration). (c) Growth curve of engrafted tumors in nude mice injected with A549 cells pretransfected (48 h before injection) with miR-29s, scrambled oligonucleotides, or mock transfected. (d) Comparison of tumor engraftment sizes of mock-, scrambled-, and miR-29s-transfected A549 cells 21 days after injection in nude mice. The images show average-sized tumors from among five of each category. (e) Tumor weights ± SD in nude mice.