Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1997 Mar 4;94(5):1840–1845. doi: 10.1073/pnas.94.5.1840

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 1997, The National Academy of Sciences of the USA

PMC Copyright notice

Characterization of ATM protein antiserum pAb 132. (A) Lysates from E. coli transformed with the plasmids pGEX AT-6 (lane 1) and pGEX AT-4 (lane 2) following fusion protein induction and untransformed E. coli (lane 3) were subjected to SDS/PAGE and Coomassie blue staining. Migration of the specific GST–AT fusion proteins are indicated by arrowheads. (B) Extracts shown in A were immunoblotted with pAb 132. (C) A total of 50 μg of B-310 (lane 1, wild type for ATM) and AT24RM (lane 2, homozygous mutant for ATM) cell extracts were immunoblotted with pAb 132. (D) A total of 50 μg of SDS-lysate from B-310 lymphoblasts (lane 1) and A-T patient lines AT29RM (lane 2), AT22RM (lane 3), and IARC12/AT3 (lane 4) were immunoblotted with pAb 132. (E) Blot displayed in D was stripped and reprobed with an antisera to NuMA.