Figure 3.

Analysis of ATM protein in fractionated human lymphoblasts and peripheral blood lymphocytes. (A) B-310 lymphoblasts (1 × 10⁷) were fractionated. Ten percent of the resultant nuclear (lane 1), microsomal (lane 2), and cytoplasmic (lane 3) fractions were immunoblotted with pAb 132 (Top). To evaluate purity of the preparations, 2% of each fraction was analyzed with NuMA antisera (Middle), or 1% of each fraction was analyzed with anti-tubulin (Bottom). (B) Normal peripheral blood lymphocytes (2 × 10⁸) were fractionated and analyzed as in A.