Fig. 2.

GUS expression from the NTH15 upstream region in tobacco and the determination of the transcription initiation sites. (A–C) GUS expression in transgenic tobacco seedlings harboring NTH15p–GUS, ΔK-GUS, and ΔRB-GUS transgenes were detected, respectively. Arrows indicate ectopic expression of GUS proteins in leaves. (D and E) GUS expression in transverse sections of leaves of transgenic tobacco harboring NTH15p–GUS and ΔK-GUS transgenes are shown, respectively. (F and G) Agarose gel electrophoresis of nested PCR products from the RLM-RACE procedure for endogenous NTH15 gene and GUS reporter transgenes, respectively. Molecular size markers (base pairs) are shown on the left. Arrows on the right mark the major PCR products. (H) Sequence around the core K-box of the NTH15 gene is illustrated, and arrows above the sequence indicate the identified transcription start sites. Horizontal arrow below the sequence indicates the core K-box. (Scale bars: A–C, 2 mm; D and E, 0.4 mm.)