Fig. 2.

The Ikkα ^AA mutation reduces cyclin D1 expression in MMTV-c-neu mammary cancers. (A) Tumor histology. (Magnification: ×20.) (B) Average tumor numbers per mouse in Ikkα ^+/+/MMTV-c-neu and Ikkα ^AA/AA/MMTV-c-neu mice. Tumors were excised from mice of indicated genotypes, paraffin-embedded, sectioned, and H&E-stained. (C) Cyclin D1 expression in primary cancer cell cultures kept with (+) or without (−) 10% serum and 10 ng/ml EGF for 2 days before analyses. (D) Cyclin D1 mRNA levels in Ikkα^+/+/MMTV-c-neu (WT) and Ikkα ^AA/AA/MMTV-c-neu (AA) tumors and primary tumor cell cultures.