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Preneoplastic changes in MMTV-c-neu transgenic mice. (A) Mammary glands of 5-month-old Ikkα+/+/MMTV-c-neu and Ikkα AA/AA/MMTV-c-neu female mice were fixed for whole-mount staining (Upper) and H&E staining (Lower). (B) Immunohistochemical staining for neu/ErbB2 expression on paraffin sections. (C) Immunofluorescence staining for BrdU incorporation in the same samples as A and B. Mice were injected with BrdU 2 h before analysis. (Magnification: ×10.)
