Fig. 5.
Effect of sucrose treatment on wild-type and cyp38-2 plants. (A) Wild-type and cyp38-2 plants were grown vertically on half-strength MS medium without sucrose for 4 weeks under low-light conditions (25–35 μmol·m−2·s−1). (B) Wild-type and cyp38-2 plants were grown vertically on half-strength MS medium supplemented with 2% sucrose under low-light conditions (25–35 μmol·m−2·s−1) for 4 weeks. (C) Plants grown as described in B were exposed to high light (300 μmol·m−2·s−1) for 5 days. (D) BN/PAGE of thylakoid membrane complexes from wild-type (WT) and cyp38-2 plants after 4 weeks of growth on 2% sucrose half-width MS medium. PSI-M, PSI monomer; PSII-D, PSII dimer. (E) Immunoanalysis of thylakoid proteins (1 μg of chlorophyll) from wild-type total protein (WTT), cyp38-2 total protein (38-2T), wild-type membrane protein (WTM), and cyp38-2 membrane protein (38-2M). Plants were grown on 2% sucrose medium for 4 weeks.