Fig. 7.

Half-life of thylakoid membrane proteins in wild-type and cyp38 plants. (A) Arabidopsis leaves from sucrose-grown wild-type (WT) and cyp38-2 plants were incubated with lincomycin and cycloheximide for 30 min and illuminated under 25–35 μmol·m⁻²·s⁻¹ for 0, 2, and 4 h. Thylakoid membrane proteins (1 μg of chlorophyll) were resolved by SDS/PAGE, and the gel was immunoblotted to assess protein half-life. (B) Conditions as in A, except that leaves were illuminated under 300 μmol·m⁻²·s⁻¹ for 0 and 2 h.