Fig. 5.

Enhanced bacterial growth and increased bacterial escape from PKCδ^−/− phagosomes. WT and PKCδ^−/− BMDMs were activated overnight with IFN-γ and infected with LM. (A) Bacterial load was determined 2, 4, 8, and 12 h p.i. (***, P < 0.0001). (B) Quantification of LM escape from phagosomes at 90, 180, and 270 min p.i. The number of bacteria in the cytoplasm associated with actin (red) and trapped in the phagosome (green) was scored. Data shown are averages ± SEM of the means for three independent experiments (*, P < 0.05). (C) Deconvoluting fluorescent microscopy of BMDMs infected at 90 min with LM. Escaped bacteria (orange/yellow) are indicated by arrows. (Magnification: ×1,000.) All results are representative of three independent experiments.