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. Author manuscript; available in PMC: 2007 Nov 28.
Published in final edited form as: J Biol Chem. 2002 Apr 15;277(26):23131–23136. doi: 10.1074/jbc.M201530200

Table III. Ecto-phosphatases mRNA expression in 3T3F442A preadipocytes and adipocytes, and mouse perigonadic isolated adipocytes.

Cells and mRNA extract were obtained as described in Experimental Procedures. mRNA levels of the known ecto-phosphatases were assessed by real-time quantitative RT-PCR as described in Experimental Procedures. The expression of each gene was quantified and normalised using simultaneous amplification of known quantities of the corresponding cDNA and determination of the concentration of 18 S in each sample. Results represent means ± sem from four different RNA preparations.

mRNA 10 −7 ng / 25 ng RNA
3T3F442A
Preadipocyte Adipocyte Perigonadic mouse tissue
LPP1 28 ± 5 12 ± 3 12 ± 1
LPP2 8 ± 1 2 ± 1 0.5 ± 0.1
LPP3 37 ± 8 10 ± 3 36 ± 4