Table IV. Ecto-phosphatase specific activity determined on intact 3T3F442A preadipocytes and adipocytes or mouse perigonadic isolated adipocytes.
Cells obtained as described in Experimental Procedures were incubated in the presence of 5 μM [33P]-LPA for 10 min, then water soluble [33P] was measured and enzyme specific activity determined. Results represent means ± sem from three separated experiments.
| Specific Activity nmoles / min / mg of protein | |
|---|---|
| 3T3F442A | |
| Preadipocyte | 0.863 ± 0.161 |
| Adipocyte | 0.178 ± 0.005 |
| Perigonadic adipose tissue | 0.263 ± 0.017 |