Expression of cell cycle-regulated genes in E2f2-deleted cells. (A) Real-time RT-PCR analysis of MYC, ODC, p19ARF, p73, p21Cip1, and p27Kip1 expression in normal thymocytes (E2f2+/+), E2f2-deficient thymocytes (E2f2−/−), tumors derived from EμSR-tTA;Teto-MYC;E2f2+/+ or EμSR-tTA;Teto-MYC;E2f2−/− mice, as indicated. Sequences of primers used for tested genes are shown in SI Table 1. (B) Western blot analysis of p27Kip1 expression in normal thymocytes (N) and EμSR-tTA;Teto-MYC tumors nondeleted or deleted for E2f2 as indicated.